3D CelluSponge
Features

 
  • 3D HPC scaffold

       - Designed for cells requiring no specific ligands and various cell lines.

 

  • Advantages of CelluSponge Series

       - Natural source.

       - Spheroid size is controllable and under 200 μm.

       - Seeding the same as in 2D cell culture and is required no extra steps.

       - Cells grow rapidly with functions.

       - Compatible to automatic equipment.

 

Description

  • Simple and efficient cell seeding procedure.
  • Cells distributing well in the CelluSponge pores and grow well easily
  • Compatible with well plate and insert.
  • Exclusive patent license-Hydrogel-like microporous sponge.
  • Appropriate spheroid size with prevented apoptosis in the spheroids center.
  • Good for cell cryopreservation.
  • Form functional spheroids within 24 hours post-seeding and remain stable at least 40 days.

 

Specifications

  • Three types of 3D CelluSponge:

(1) 3D CelluSponge (Plain)

(2) 3D CelluSponge-Gal (Galactose)

(3) 3D CelluSponge-Coll (Collagen)

- Size: 6/ 9 mm diameter CelluSponge scaffold

- Pore size: between 100 - 200 μm (lrregular)

 

Application range

(1) 3D CelluSponge (Plain)

  • For cells requiring no specific ligand and various cancer cell lines
  • Suitable cell types:

        - Human Breast cancer cell line (MCF-7 & MDA-MB) ·

        - Mouse fibroblasts (NIH3T3)

        - Human foreskin fibroblast (HFF) 

 

 

(2) 3D CelluSponge-Gal (Galactose)

  • For Hepatocyte

       ​- Hepatocytes for 3D functional spheroids after one day of seeding and the spheroids maintained stable or at lease at 40 days and up to 100 days.

 

 

 

(3) 3D CelluSponge-Coll (Collagen)

  • For ESC,MSC, primary cells

      - HMSC cells grew 14 times aker 7 days and differentiate into neurons and glial cells after 14 days (Usually it takes 28 days).

 

Technical documents
  • References:

(1) Galactosylated cellulosic sponge for multi-well drug safetytesting.
https://www.ncbi.nlm.nih.gov/pubmed/?term=Galactosylated+cellulosic+sponge+for+multi-well+drug+safety+testing

(2) Preparation of three-dimensional interconnected macroporouscellulosic hydrogels for soft tissue engineering
https://www.ncbi.nlm.nih.gov/pubmed/?term=Preparation+of+three-dimensional+interconnected+macroporous+cellulosic+hydrogels+for+soft+tissue+engineering

(3) Control of in vitro neural differentiation of mesenchymal stem cells in 3D macroporouscellulosic hydrogels
https://www.ncbi.nlm.nih.gov/pubmed/20210584


(4) Functionally Enhanced Human Stem Cell Derived Hepatocytes in Galactosylated Cellulosic Sponges for Hepatotoxicity Testing.
http://pishonmd.com/PDF/Functionally%20Enhanced%20Human%20Stem%20Cell%20Derived%20Hepatocytes%20in%20Galactosylated%20Cellulosic%20Sponges%20for%20Hepatotoxicity%20Testing.pdf

(5) Scalable Spheroid Model of Human Hepatocytes for Hepatitis C Infection and Replication.
http://pishonmd.com/PDF/Scalable%20Spheroid%20Model%20of%20Human%20Hepatocytes%20for%20Hepatitis%20C.pdf

 

  • Cellusponge Cell Seeding Protocol​

http://www.bbbmd.com/en/products/files/update_Cellusponge_Cell%20Seeding%20Protocol.pdf

  • Product Introduction Video

https://www.youtube.com/watch?v=2yoVSVedPiM

  • Product Video

https://www.youtube.com/watch?v=bK2qh6sihTQ

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