3D CelluSponge

  • 3D HPC scaffold

       - Designed for cells requiring no specific ligands and various cell lines.


  • Advantages of CelluSponge Series

       - Natural source.

       - Spheroid size is controllable and under 200 μm.

       - Seeding the same as in 2D cell culture and is required no extra steps.

       - Cells grow rapidly with functions.

       - Compatible to automatic equipment.



  • Simple and efficient cell seeding procedure.
  • Cells distributing well in the CelluSponge pores and grow well easily
  • Compatible with well plate and insert.
  • Exclusive patent license-Hydrogel-like microporous sponge.
  • Appropriate spheroid size with prevented apoptosis in the spheroids center.
  • Good for cell cryopreservation.
  • Form functional spheroids within 24 hours post-seeding and remain stable at least 40 days.



  • Three types of 3D CelluSponge:

(1) 3D CelluSponge (Plain)

(2) 3D CelluSponge-Gal (Galactose)

(3) 3D CelluSponge-Coll (Collagen)

- Size: 6/ 9 mm diameter CelluSponge scaffold

- Pore size: between 100 - 200 μm (lrregular)


Application range

(1) 3D CelluSponge (Plain)

  • For cells requiring no specific ligand and various cancer cell lines
  • Suitable cell types:

        - Human Breast cancer cell line (MCF-7 & MDA-MB) ·

        - Mouse fibroblasts (NIH3T3)

        - Human foreskin fibroblast (HFF) 



(2) 3D CelluSponge-Gal (Galactose)

  • For Hepatocyte

       ​- Hepatocytes for 3D functional spheroids after one day of seeding and the spheroids maintained stable or at lease at 40 days and up to 100 days.




(3) 3D CelluSponge-Coll (Collagen)

  • For ESC,MSC, primary cells

      - HMSC cells grew 14 times aker 7 days and differentiate into neurons and glial cells after 14 days (Usually it takes 28 days).


Technical documents
  • References:

(1) Galactosylated cellulosic sponge for multi-well drug safetytesting.

(2) Preparation of three-dimensional interconnected macroporouscellulosic hydrogels for soft tissue engineering

(3) Control of in vitro neural differentiation of mesenchymal stem cells in 3D macroporouscellulosic hydrogels

(4) Functionally Enhanced Human Stem Cell Derived Hepatocytes in Galactosylated Cellulosic Sponges for Hepatotoxicity Testing.

(5) Scalable Spheroid Model of Human Hepatocytes for Hepatitis C Infection and Replication.


  • Cellusponge Cell Seeding Protocol​


  • Product Introduction Video


  • Product Video